Extraction, Fractionation, Phytochemical Profile, Antioxidant, and Antimicrobial Activities of Cassia absus L. and Citrus medica L.

Background: Cassia absus L. and Citrus medica plants are sources of different phytochemicals with pharmaceutical significance. They biochemically distinctive used in producing various herbal products exclusive bioactivity. Also, the extraction these phytonutrients medicinal characteristics has recently increased. Objectives: We determined to evaluate plant-derived compounds such as polyphenols antioxidant antimicrobial activity methanolic extract Methods: Antioxidant was calculated through linoleic acid inhibition, determination reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The disk diffusion method for assessing activity. Various solvents acetic acid, ethanol, acetone, n-hexane, ethyl acetate were perform partition fractionation plants. Results: Three major components analyzed high-performance liquid chromatography C. medica. Total phenolic contents varied from 5.58-17.1 2.66-7.41 per 100 g examined Folin-Ciocalteu reagent, pointed gallic equivalents (GAE), total flavonoid lined 11.50-32.4 24.91-36.36 specified catechin equivalents. Inhibition rate potential greater fractions 49.6% 1.14, respectively. solvent exhibited radical DPPH assay at 80%. significantly inhibit gram-negative (Escherichia coli, Pasteurella multocida, Fusarium) gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, Aspergillus flavus, niger, Fusarium Solani) bacteria. Conclusion: Both leaf extracts could have applications agriculture, pharmaceutics, medicine.